Anti-Human Desmoglein2 (Clone 141409)

Anti-Human Desmoglein-2 (Clone 141409)

Product No.: D356


Clone 141409 Target Desmoglein-2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names ARVC10, ARVD10, CDHF5, HDGC, MGC117034, MGC117036, MGC117037 Isotype IgG1 Applications ELISA Indirect , ICC , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human Desmoglein-2 (Accession # CAA81226) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829936 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human Desmoglein-2 is approximately 0.5 ng/well. Western Blotting: To detect Human Desmoglein-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Desmoglein-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Immunocytochemistry: Suitable for use at concentration of 5-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Desmoglein-2 (DSG2) (Clone 141409) recognizes an epitope on Human DSG2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Desmoglein 2, also known as DSG2, is a human gene. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It provides cellular adhesion and forces transduction by cell-to-cell anchorage. ¹ It also plays a role in embryonic stem cell proliferation. PubMed Desmoglein-2 NCBI Gene Bank ID 1829 UniProt.org More Information on Uniprot.org References & Citations 1. Ozcelik, C. et al. (2008) Mol Genet Metab. 95(1-2):74-80. View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols ICC Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

NS0-Derived Recombinant Human Desmoglein-2 (Accession # CAA81226)

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2829936

Applications and Recommended Usage?
Quality Tested by Leinco

Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human Desmoglein-2 is approximately 0.5 ng/well.
Western Blotting: To detect Human Desmoglein-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Desmoglein-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Additional Applications Reported In Literature ?

Immunocytochemistry: Suitable for use at concentration of 5-25 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Desmoglein-2 (DSG2) (Clone 141409) recognizes an epitope on Human DSG2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Desmoglein 2, also known as DSG2, is a human gene. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It provides cellular adhesion and forces transduction by cell-to-cell anchorage. ¹ It also plays a role in embryonic stem cell proliferation.

PubMed

Desmoglein-2

NCBI Gene Bank ID

1829

UniProt.org

More Information on Uniprot.org