Anti-Human DR6/TNFRSF21

Anti-Human DR6/TNFRSF21

Product No.: D375


Target DR6 Product Type Polyclonal Antibody Alternate Names Death Receptor 6, TNFRSF21 Applications ELISA Cap , WB


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Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified NS0-derived Recombinant Human DR6 (Accession # O75509) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829953 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/ml. A suitable detection antibody is PN:D363 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:D332. Western Blotting: To detect Human DR6 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human DR6 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:332. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Goat Anti-Human Death Receptor 6 (DR6) recognizes Human DR6. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. PubMed DR6 NCBI Gene Bank ID 27242 UniProt.org More Information on Uniprot.org References & Citations 1. Na, S. et al.(2006)Immunol Lett. 106:42 2. Shi, J. et al. (2018) FEBS Open Bio. 8(9):1497-1507. View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified NS0-derived Recombinant Human DR6 (Accession # O75509)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2829953

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/ml. A suitable detection antibody is PN:D363 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:D332.
Western Blotting: To detect Human DR6 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human DR6 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:332.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Death Receptor 6 (DR6) recognizes Human DR6. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

PubMed

DR6

NCBI Gene Bank ID

27242

UniProt.org

More Information on Uniprot.org