Anti-Human E-Selectin-Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Sheep Immunogen Purified Recombinant Human E-Selectin (>98%) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830071 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human E-Selectin this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E221. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Sheep Anti-Human E-Selectin recognizes Human E-Selectin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background E-selectin, also known as CD62E is an inducible leukocyte adhesion glycoprotein specifically expressed by endothelial cells.1 E-selectin helps initiate recruitment of circulating leukocytes to cutaneous, bone and inflamed tissues.2 E-selectin is found in inflammatory skin lesions in psoriasis, contact dermatitis, and delayed- type hypersensitivity, in arthritic joints, and in heart and renal allografts undergoing rejection.3 E-selectin-targeting antibody drug conjugate has potential as a prostate cancer therapy.4 PubMed NCBI Gene Bank ID References & Citations1. Milstone, D. et al. (2004) PNAS 101: 8005 2. Dimitroff, CJ. et al. (2009) J Visualized Experiments 3. Hirata, T. et al. (2005) J Immunol. 175: 8042 4. Vanitha, R. et al. (2003) Cancer Res. 63: 6387 Technical ProtocolsCertificate of Analysis |
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