Anti-Human Ephrin-A4 (Clone 135305)
Anti-Human Ephrin-A4 (Clone 135305)
Product No.: E248
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Clone 135305 Target Ephrin-A4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names LERK-4, EFL-4, EFNA4, EPLG4, MGC125826 Isotype IgG2b Applications IHC , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human Ephrin-A4 (Accession # P52798) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830020 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human Ephrin-A4 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Ephrin-A4 is 5.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E199. Additional Applications Reported In Literature ? Immunohistochemistry: Suitable for use at concentration of 5-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human Ephrin-A4 (Clone 135305) recognizes an epitope on Human Ephrin-A4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Ephrin-A4 is a glycoinositol phospholipid membrane anchor containing ephrin found in variety of adult tissue such as kidney, lung, colon and ovary. Ephrin-A4 is a ligand for the erythropoietin-producing hepatocellular (Eph) receptor family of tyrosine kinases.1 Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin-A4 encodes an EFNA class ephrin. Ephrin-A4 (s) may play a role in the interaction between activated B lymphocytes and dendritic cells in human tonsils. PubMed References & Citations1. Logtenberg, T. et al. (2000) Blood 95: 221 Technical Protocols  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
