Anti-Human GDF-15
Anti-Human GDF-15
Product No.: G667
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Target GDF-15 Product Type Polyclonal Antibody Alternate Names Growth Differentiation Factor 15, Macrophage Inhibitory Cytokine-1, MIC-1, Placental TGFβ, Prostate Differentiation Factor, PDF, GDF-15, NAG-1, PLAB, PTGFB Applications WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen CHO Cell-Derived Recombinant Human GDF-15 Endotoxin Level <1.0 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830257 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human GDF-15 this polyclonal antibody can be used at a concentration of 0.5 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human GDF-15 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Growth Differentiation Factor 15 (GDF-15) recognizes Human GDF-15. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Growth differentiation factor 15 (GDF15) is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes.1 GDF15 is also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB.2 GDF15 is predominantly expressed in the liver, with lower levels seen in some other tissues. Its expression in the liver is significantly up-regulated during injury of organs such as liver, kidney, heart and lung.1,2,3 GDF-15 is a promising new biomarker in idiopathic pulmonary arterial hypertension.4 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Koniaris, L.et al. (2005)Shock 23: 543 2. Lee, S. et al. (2000)Mol Cell Biol 20: 3742 3. Sadoshima, J. et al. (2006)Circ Res 98 : 294 4. Wollert, KC. et al. (2008)Am J Respir Crit Cre Med. 178 : 534 Technical Protocols Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
