Anti-Human GM-CSF (Clone 6804)
Anti-Human GM-CSF (Clone 6804)
Product No.: G675
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Clone 6804 Target GM-CSF Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-Alpha Isotype IgG1 Applications CyTOF® , ELISA Cap , FC |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen E. coli-Derived Recombinant Human GM-CSF (Accession # P04141) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830264 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing GM-CSF. A suggested method would be to stain cells expressing GM-CSF with 2.5 µg per 1 - 5 x 106 cells in a total staining volume of ≤200 µl followed by PN:M1189. ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. Use in combination with: Detection Reagent: Human GM‑CSF Biotinylated Antibody. Standard: Recombinant Human GM-CSF Protein. Additional Applications Reported In Literature ? CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 6804 recognizes an epitope on human GM-CSF. Background Granulocyte-Macrophage Colony Stimulating Factor is a 22 kD, pleiotropic cytokine that is a white blood cell growth factor. It controls the production and function of blood cells by stimulating stem cells to produce granulocytes and monocytes. GM-CSF differs from G-CSF in that it affects more cell types including macrophages and eosinophils. Moreover, GM-CSF is part of the immune/inflammatory cascade, a process crucial for fighting infection. Interestingly, GM-CSF expression may have pathological implications. Autocrine expression of GM-CSF in myeloid leukemia cells is suspected to play a role in neoplasia, the formation of a new and abnormal growth of tissue. Additionally, GM-CSF expression has also been documented in certain solid tumors. There have also been reports of GM-CSF in synovial fluid from patients with arthritis suggesting that GM-CSF may play a role in tissue damage associated with the inflammatory process. Blocking GM-CSF is thought to have therapeutic potential by reducing inflammation. Some drugs are currently being developed to block GM-CSF. PubMed References & Citations1. Parker, MW. et al. (2008) Cell 134: 496 2. Whitsett, JA. et al. (2002) Annual Review of Physiology 64: 775 Technical ProtocolsCyTOF®   Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
