Anti-Human Hepsin (Clone 2D5) – Purified in vivo GOLD™ Functional Grade
Anti-Human Hepsin (Clone 2D5) – Purified in vivo GOLD™ Functional Grade
Product No.: H4400
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Clone 2D5 Target Hepsin Formats AvailableView All Product Type Monoclonal Antibody Alternate Names HPN, TMPRSS1, 2D5-1.9, 2D5.1.9 Isotype Mouse IgG2a k Applications ELISA , FC , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Human hepsin protein Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2830332 Applications and Recommended Usage? Quality Tested by Leinco ELISA FC WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 2D5 recognizes Human hepsin. It binds to a separate epitope than clone 3H10.1. These clones do not interfere with one another. Background Hepsin is a type II transmembrane serine protease (TTSP) expressed on the surface of epithelial cells including the liver, kidney, prostate, and thyroid in human tissues. The physiological function of hepsin is unclear, although, In vitro studies have shown that hepsin activates blood clotting factors VII, XII, and IX, pro-urokinase (pro-uPA), and pro-hepatocyte growth factor (pro-HGF). The over-expression of hepsin has been implicated in several types of cancer, especially ovarian and prostate, which makes it an attractive diagnostic marker for cancers. Most notably, hepsin has been identified as one of the most highly induced genes in prostate cancer, and this over-expression is correlated with the cancer progression and metastasis. Furthermore anti-hepsin antibodies have been shown to inhibit the invasion of human prostate cancer cells.1 Antigen Distribution Hepsin is expressed on the surface of epithelial cells including the liver, kidney, prostate, and thyroid in human tissues. PubMed NCBI Gene Bank ID UniProt.org Research Area Immuno-Oncology References & Citations1. Wu, Q. and Parry, G. (2007) Front Biosci 12 5052-9 Technical Protocols   Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
