Anti-Human IκB Kinase Alpha (CT-1) (IKKα)

PN:I-313

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

Rabbit Anti-Human IκB Kinase Alpha (IKKα) recognizes an epitope near the C-terminus of Human IKKα. This polyclonal antibody was purified using affinity chromatography.

Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories.^1-4 IKK is a serine protein kinase, and the IKK complex contains α and β subunits (IKKα and IKKβ). IKKa and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IkB-α. IKKα is expressed in variety of human tissues.

1. DiDonato, JA. et al. (1997) Nature 388:548
2. Regnier, CH. et al. (1997) Cell 90:373
3. Zandi, E. et al. (1997) Cell 91:243
4. Woronicz, JD. et al. (1997) Science 278:866