Anti-Human ICAM-1 – FITC

Purified Recombinant Human ICAM-1 (>98%)

This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.

Next Day Ambient

Blue Laser (490 nm)

Mouse Anti-Human CD54 (Clone 15.2) recognizes Human CD54. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD54 (ICAM-1) recognizes the (Mr 85-115 kDa) intercellular adhesion molecule ICAM-1 which permits antigen-independent adhesion between lymphocytes and their targets. The LFA-1 binding site is located in domain 1 of ICAM-1.^1,2

Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein¹ and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines.² ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.³

ICAM-1 is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes. CD54 is the receptor for rhinoviruses and malaria.¹

1. Li S et al. (2009) Biochem Biophys Res Commun. 381: 459 2. Wolf S et al. (2009) Pharmacol Rep 61: 22 3. Ozcan U et al. (2009) Arch Gynecol Obstet.