Anti-Human IFN-γ

Purified Recombinant Human IFN-γ (Accession # AAP20098.1)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

NThis antibody is useful for neutralization of Human IFN-γ bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IFN-γ (at 50 ng/ml) is 0.02 - 0.06 µg/ml.
ICC Immunocytochemistry suitable for use at concentration of 8-25 µg/mL.

Mouse Anti-Human Interferon Gamma (IFN-γ) (Clone 25718) recognizes an epitope on Human IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.¹ It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.² IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.³

1. Goeddel, DV. et al. (1982) Nature 298: 859
2. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41
3. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163