Anti-Human IGF-I R

Anti-Human IGF-I R

Product No.: I-657


Clone 33255 Target IGF-I R Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Insulin-Like Growth Factor I Receptor Isotype IgG1 Applications ELISA Cap , FC , ICC , IHC FFPE , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human IGF-I R (Accession # P08069) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830779 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-771 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-841. Western Blotting: To detect Human IGF-I R this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IGF-I R is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-841. Flow Cytometry: Suitable for use at concentration of 0.25 µg/10⁶ cells. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 - 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This anti-human IGF-I R antibody will neutralize approximately 50 - 75% of IGF-I R-mediated rhIGF-I biological activity on MCF-7 cells. Immunocytochemistry: Suitable for use at concentration of 3-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Human Insulin-Like Growth Factor I Receptor (IGF-I R) (Clone 33255) recognizes an epitope on Human IGF-I R. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed IGF-I R References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols ICC IHC FFPE N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human IGF-I R (Accession # P08069)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830779

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-771 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-841.
Western Blotting: To detect Human IGF-I R this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IGF-I R is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-841.
Flow Cytometry: Suitable for use at concentration of 0.25 µg/10⁶ cells.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 - 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This anti-human IGF-I R antibody will neutralize approximately 50 - 75% of IGF-I R-mediated rhIGF-I biological activity on MCF-7 cells.
Immunocytochemistry: Suitable for use at concentration of 3-25 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Insulin-Like Growth Factor I Receptor (IGF-I R) (Clone 33255) recognizes an epitope on Human IGF-I R. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

PubMed

IGF-I R