Anti-Human IGFBP-2
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen NS0-Derived Recombinant Human IGFBP-2 Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830852 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human IGFBP-2 this polyclonal antibody can be used at a concentration of 0.5 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IGFBP-2 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human IGFBP-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IGFBP-2 (at 14 ng/ml) is 2.5 - 7.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Insulin-Like Growth Factor Binding Protein 2 (IGFBP-2) recognizes Human IGFBP-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Insulin-like growth factor binding protein 2, also known as IGFBP2, is an extracellular unglycosylated polypeptide that specifically binds IGF and modulates its effects.1 IGFBP-2 has direct effects on breast cancer proliferation via stimulation of critical signaling pathways.2 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Luthman, H. et al.(1991) Biochem Biophys Res Commun. 176: 1250 2. Disis, ML. et al.(2008) Cancer Res. 68: 8400 Technical ProtocolsN  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
