Anti-Human IGFBP-2 (Clone 92326)
Anti-Human IGFBP-2 (Clone 92326)
Product No.: I-694
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Clone 92326 Target IGFBP-2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Insulin-Like Growth Factor Binding Protein 2, IBP-2,IGF-BP53 Isotype IgG1 Applications ELISA Cap , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human IGFBP-2 (Accession # CAA34373) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830815 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-791 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-375. Western Blotting: To detect Human IGFBP-2 this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IGFBP-2 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-375. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human Insulin-Like Growth Factor Binding Protein 2 (IGFBP-2) (Clone 92326) recognizes an epitope on Human IGFBP-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Insulin-like growth factor binding protein 2, also known as IGFBP2, is an extracellular unglycosylated polypeptide that specifically binds IGF and modulates its effects.1 IGFBP-2 has direct effects on breast cancer proliferation via stimulation of critical signaling pathways.2 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Luthman, H. et al.(1991) Biochem Biophys Res Commun. 176: 1250 2. Disis, ML. et al.(2008) Cancer Res. 68: 8400 Technical Protocols  Certificate of Analysis |
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I-791 |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
