Anti-Human IgM µ-Chain Specific – HRP
Anti-Human IgM µ-Chain Specific – HRP
Product No.: I-1201
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Clone HB57 Target IgM µ-Chain Specific Formats AvailableView All Product Type Monoclonal Antibody Isotype IgG1 Applications ELISA , FC |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Product Concentration 0.5 mg/ml Formulation This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. <b> (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)</b> Storage and Handling This conjugated antibody is stable when stored at 2-8°C. Do not freeze. Cross Reactivity Cross-reactivity by ELISA against human myeloma proteins: Human IgG1: 100% Human IgG2: 100% Human IgG3: 100% Human IgG4: 100% Human IgM: <0.2% Human IgA: <0.1% Human IgE: <0.1% There is no detectable binding to bovine, goat, horse sheep IgG. NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HB57) when conjugated to HRP is validated for use in Enzyme Immunoassay for the detection of Human IgG, µ-Chain Specific (Part No.: I-1201) Country of Origin USA Shipping Next Day Ambient RRIDAB_2893815 Applications and Recommended Usage? Quality Tested by Leinco FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Anti-Human IgM (µ chain specific) monoclonal antibody reacts with human IgM through an epitope on the heavy chain. This antibody is non-reactive with IgG, IgA or light chains. Clone HB57 produces an antibody with one of the highest affinities available (Ka = 5.34 x 108 M-1).1 Antigen Distribution Surface IgM is expressed on B-lymphocytes. PubMed References & Citations1. Rudich, S. M. et al. (1988) J. Exp. Med. 168:247
Technical Protocols  Certificate of Analysis |
Formats Available
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Prod No. | Description |
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I-995 | |
I-987 | |
I-988 | |
I-989 | |
I-991 | |
I-992 | |
I-993 | |
I-121 | |
I-1202 | |
I-122 | |
I-123 | |
I-1201 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
