Anti-Human IgM µ-Chain Specific – Purified in vivo GOLD™ Functional Grade
Anti-Human IgM µ-Chain Specific – Purified in vivo GOLD™ Functional Grade
Product No.: I-1202
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Clone HB57 Target IgM µ-Chain Specific Formats AvailableView All Product Type Monoclonal Antibody Isotype Mouse IgG1 Applications ELISA , FC , in vivo |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Cross Reactivity Cross-reactivity by ELISA against human myeloma proteins: Human IgG1: 100% Human IgG2: 100% Human IgG3: 100% Human IgG4: 100% Human IgM: <0.2% Human IgA: <0.1% Human IgE: <0.1% There is no detectable binding to bovine, goat, horse sheep IgG. NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HB57) when conjugated to HRP is validated for use in Enzyme Immunoassay for the detection of Human IgG, µ-Chain Specific (Part No.: I-1201) Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2893816 Applications and Recommended Usage? Quality Tested by Leinco FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Anti-Human IgM (µ chain specific) monoclonal antibody reacts with human IgM through an epitope on the heavy chain. This antibody is non-reactive with IgG, IgA or light chains. Clone HB57 produces an antibody with one of the highest affinities available (Ka = 5.34 x 108 M-1).1 Antigen Distribution Surface IgM is expressed on B-lymphocytes. PubMed References & Citations1. Rudich, S. M. et al. (1988) J. Exp. Med. 168:247
Technical Protocols   Certificate of Analysis |
Formats Available
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Prod No. | Description |
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I-995 | |
I-987 | |
I-988 | |
I-989 | |
I-991 | |
I-992 | |
I-993 | |
I-121 | |
I-1202 | |
I-122 | |
I-123 | |
I-1201 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
