Anti-Human IL-10

Purified Recombinant Human IL-10 (>98%)

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. The Neutralization Dose (ND₅₀) is typically 0.2-1.0 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑10.
Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL.

Rabbit Anti-Human Interleukin-10 (IL-10) recognizes Human IL-10. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is a homodimer protein produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and the Type 1 T helper cells. IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. It is released by cytotoxic T-cells to inhibit the actions of NK cells during the immune response to viral infection. IL-10 plays a key role in peripheral T cell tolerance to allergens, autoantigens, transplantation antigens and tumor antigens.¹

1. Akdis, CA. et al. (2009) Mol. Immunol. 46: 622
2. Medzhitov, R. et al. (2017) Science. 356(6337):513-519.