Anti-Human IL-10 Rβ

Anti-Human IL-10 Rβ

Product No.: I-748


Target IL-10 R beta Product Type Polyclonal Antibody Alternate Names Interleukin-10 Receptor Beta, IL10RB, CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2 Applications CyTOF® , FC , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen NS0-Derived Recombinant Human Interleukin-10 Receptor β Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830869 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human IL-10 Rβ this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The sensitivity for rhIL-10 Rβ is approximately 5 ng/lane and 2 ng/lane under non-reducing and reducing conditions, respectively. Flow Cytometry: Suitable for use at concentration of 2.5 µg/10⁶cells Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human IL-10 Rβ bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-10 Rβ (at 0.25 ng/ml) is 2 - 6 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Human Interleukin 10 Receptor Beta (IL-10 Rβ) recognizes Human IL-10 Rβ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Interleukin 10 receptor, beta subunit (IL10RB) is a subunit for the interleukin-10 receptor which has recently been designated CDW210B (cluster of differentiation W210B). The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. PubMed IL-10 R beta NCBI Gene Bank ID 3588 UniProt.org More Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols CyTOF® N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

NS0-Derived Recombinant Human Interleukin-10 Receptor β

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830869

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human IL-10 Rβ this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The sensitivity for rhIL-10 Rβ is approximately 5 ng/lane and 2 ng/lane under non-reducing and reducing conditions, respectively.
Flow Cytometry: Suitable for use at concentration of 2.5 µg/10⁶cells

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human IL-10 Rβ bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-10 Rβ (at 0.25 ng/ml) is 2 - 6 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Interleukin 10 Receptor Beta (IL-10 Rβ) recognizes Human IL-10 Rβ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Interleukin 10 receptor, beta subunit (IL10RB) is a subunit for the interleukin-10 receptor which has recently been designated CDW210B (cluster of differentiation W210B). The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction.

PubMed

IL-10 R beta

NCBI Gene Bank ID

3588

UniProt.org

More Information on Uniprot.org