Anti-Human IL-11 (Clone 22616)

Product No.: I-153


Clone 22616 Target IL-11 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-11, AGIF(Adipogenesis Inhibitory Factor) Isotype IgG2b Applications ELISA Cap , WB


Select Product Size 500 µg — $0 100 µg — $500.00 25 µg — $250.00	Qty Max: Min: 1 Step: 1 Select A Size ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human IL-11 (Accession # P20809) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830547 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 - 8 µg/ml. A suitable detection antibody is PN:I-266 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-194. Western Blotting: To detect Human IL-11 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-11 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-194. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Interleukin-11 (IL-11) (Clone 22616) recognizes an epitope on Human IL-11. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows 100% cross-reactivity with rmIL-11 and no cross-reactivity with rhIL-6, rhIL-6 R, rhLIF, rhOSM, rhCT-1, rhCNTF, or rhCLC. Background Interleukin 11 (IL-11) is a multifunctional cytokine isolated from bone marrow-derived stromal cells. It is a key regulator of multiple events in hemtaopoiesis, most notably the stimulation of megakaryocyte maturation.¹ It is also known under the names Adipogenesis inhibitory factor (AGIF)² and Oprelvekin. IL-11 improves platelet recovery after chemotherapy-induced thrombocytopenia, induces acute phase proteins, modulates antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. Besides from lymphopoietic/hematopoietic and osteotrophic properties, it has functions in many tissues such as brain, gut and testis. PubMed IL-11 References & Citations 1. Williams, DA. et al. (1990) Proc. Natl. Acad. Sci. USA87: 7512 2. Takiguchi, Y. et al. (1991) FEBS Lett. 283: 199 View product citations for antibody I-153 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human IL-11 (Accession # P20809)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830547

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 - 8 µg/ml. A suitable detection antibody is PN:I-266 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-194.
Western Blotting: To detect Human IL-11 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-11 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-194.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Interleukin-11 (IL-11) (Clone 22616) recognizes an epitope on Human IL-11. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows 100% cross-reactivity with rmIL-11 and no cross-reactivity with rhIL-6, rhIL-6 R, rhLIF, rhOSM, rhCT-1, rhCNTF, or rhCLC.

Background

Interleukin 11 (IL-11) is a multifunctional cytokine isolated from bone marrow-derived stromal cells. It is a key regulator of multiple events in hemtaopoiesis, most notably the stimulation of megakaryocyte maturation.¹ It is also known under the names Adipogenesis inhibitory factor (AGIF)² and Oprelvekin. IL-11 improves platelet recovery after chemotherapy-induced thrombocytopenia, induces acute phase proteins, modulates antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. Besides from lymphopoietic/hematopoietic and osteotrophic properties, it has functions in many tissues such as brain, gut and testis.

PubMed

IL-11

References & Citations

1. Williams, DA. et al. (1990) Proc. Natl. Acad. Sci. USA87: 7512
2. Takiguchi, Y. et al. (1991) FEBS Lett. 283: 199

View product citations for antibody I-153 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
I-153	Anti-Human IL-11 (Clone 22616)
I-194	Recombinant Human IL-11
I-266	Anti-Human IL-11 – Biotin
I-774	Anti-Mouse IL-11-Biotin
I-561	Recombinant Mouse IL-11

Formats Available

Prod No.	Description
I-153	Anti-Human IL-11 (Clone 22616)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

NEW Products!

Anti-Human IL-11 (Clone 22616)