Anti-Human IL-13 Rα2

Anti-Human IL-13 Rα2

Product No.: I-608


Target IL-13 R alpha 2 Product Type Polyclonal Antibody Alternate Names Interleukin-13 Receptor Alpha 2, IL13RA2, CD213A2, IL-13R, IL13BP Applications B , CyTOF® , FC , IHC FFPE , WB


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Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified Recombinant Human IL-13 Rα2 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830731 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-13 Rα2. A suggested method would be to stain cells expressing IL-13 Rα2 with 2.5 µg/10⁶ cells in a total staining volume of 200 µl followed by PN:G641. Western Blotting: To detect Human IL-13 Rα2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-13 Rα2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Blocking: Approximately 2 to 6 µg/ml of this antibody will block 50% of the binding of 100 ng/ml of human IL-13 to immobilized human IL-13 Rα2/Fc Chimera (100 µl of a 4 µg/ml solution was coated in each well) in a functional ELISA. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Human Interleukin-13 Receptor Alpha 2 (IL-13 Rα2) recognizes Human IL-13 Rα2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Interleukin 13 receptor alpha 2, also known as IL13RA2 and CD213A2, binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. IL13RA2 serves as a dominant negative inhibitor or a decoy receptor for IL-13.¹ IL-13Ralpha2 is a promising target for ovarian cancer therapy, and the soluble form of IL-13R may be a possible surrogate marker for disease monitoring.² IL13RA2 signaling during prolonged inflammation is also an important therapeutic target for the prevention of TGF-beta(1)-mediated fibrosis.³ PubMed IL-13 R alpha 2 NCBI Gene Bank ID 3598 UniProt.org More Information on Uniprot.org References & Citations 1. Low, WC. et al. (2003) Neuro Oncol. 5: 179 2. Puri, RK. et al. (2006) Cancer 107: 1407 3. Kitani, A. et al. (2006) Nat Med. 12: 99 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols B CyTOF® IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human IL-13 Rα2 (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830731

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-13 Rα2. A suggested method would be to stain cells expressing IL-13 Rα2 with 2.5 µg/10⁶ cells in a total staining volume of 200 µl followed by PN:G641.
Western Blotting: To detect Human IL-13 Rα2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-13 Rα2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Blocking: Approximately 2 to 6 µg/ml of this antibody will block 50% of the binding of 100 ng/ml of human IL-13 to immobilized human IL-13 Rα2/Fc Chimera (100 µl of a 4 µg/ml solution was coated in each well) in a functional ELISA.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Interleukin-13 Receptor Alpha 2 (IL-13 Rα2) recognizes Human IL-13 Rα2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Interleukin 13 receptor alpha 2, also known as IL13RA2 and CD213A2, binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. IL13RA2 serves as a dominant negative inhibitor or a decoy receptor for IL-13.¹ IL-13Ralpha2 is a promising target for ovarian cancer therapy, and the soluble form of IL-13R may be a possible surrogate marker for disease monitoring.² IL13RA2 signaling during prolonged inflammation is also an important therapeutic target for the prevention of TGF-beta(1)-mediated fibrosis.³

PubMed

IL-13 R alpha 2

NCBI Gene Bank ID

3598

UniProt.org

More Information on Uniprot.org