Anti-Human IL-13 Rα2 (Clone 83834)

Anti-Human IL-13 Rα2 (Clone 83834)

Product No.: I-688

[product_table name="All Top" skus="I-688"]

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Clone
83834
Target
IL-13 R alpha 2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Interleukin-13 Receptor Alpha 2, IL13RA2, CD213A2, IL-13R, IL13BP
Isotype
IgG1
Applications
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
NS0-Derived Recombinant Human Interleukin-13 Receptor α2 Extracellular Domain (Accession # Q14627)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human IL-13 Rα2 this monoclonal antibody can be used at a concentration of 5 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rhIL-13 Rα2 is approximately 25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Anti-Human Interleukin-13 Receptor Alpha 2 (IL-13 Rα2) (Clone 83834) recognizes an epitope on Human IL-13 Rα2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhIL-5 Rα, rhIL-5 Rβ, rhIL-13 Rα1 and rmIL-13 Rα2 and approximately 5% cross-reactivity with rhIL-4 R and rhIL-9 R.
Background
Interleukin 13 receptor alpha 2, also known as IL13RA2 and CD213A2, binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. IL13RA2 serves as a dominant negative inhibitor or a decoy receptor for IL-13.1 IL-13Ralpha2 is a promising target for ovarian cancer therapy, and the soluble form of IL-13R may be a possible surrogate marker for disease monitoring.2 IL13RA2 signaling during prolonged inflammation is also an important therapeutic target for the prevention of TGF-beta(1)-mediated fibrosis.3

References & Citations

1. Low, WC. et al. (2003) Neuro Oncol. 5: 179
2. Puri, RK. et al. (2006) Cancer 107: 1407
3. Kitani, A. et al. (2006) Nat Med. 12: 99
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
I-688
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.