Anti-Human IL-13 Rα2 (Clone 83834)
Anti-Human IL-13 Rα2 (Clone 83834)
Product No.: I-688
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Clone 83834 Target IL-13 R alpha 2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-13 Receptor Alpha 2, IL13RA2, CD213A2, IL-13R, IL13BP Isotype IgG1 Applications WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human Interleukin-13 Receptor α2 Extracellular Domain (Accession # Q14627) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830809 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human IL-13 Rα2 this monoclonal antibody can be used at a concentration of 5 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rhIL-13 Rα2 is approximately 25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human Interleukin-13 Receptor Alpha 2 (IL-13 Rα2) (Clone 83834) recognizes an epitope on Human IL-13 Rα2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhIL-5 Rα, rhIL-5 Rβ, rhIL-13 Rα1 and rmIL-13 Rα2 and approximately 5% cross-reactivity with rhIL-4 R and rhIL-9 R. Background Interleukin 13 receptor alpha 2, also known as IL13RA2 and CD213A2, binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. IL13RA2 serves as a dominant negative inhibitor or a decoy receptor for IL-13.1 IL-13Ralpha2 is a promising target for ovarian cancer therapy, and the soluble form of IL-13R may be a possible surrogate marker for disease monitoring.2 IL13RA2 signaling during prolonged inflammation is also an important therapeutic target for the prevention of TGF-beta(1)-mediated fibrosis.3 PubMed References & Citations1. Low, WC. et al. (2003) Neuro Oncol. 5: 179 2. Puri, RK. et al. (2006) Cancer 107: 1407 3. Kitani, A. et al. (2006) Nat Med. 12: 99 Technical ProtocolsCertificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.