Anti-Human IL-18 Rβ
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human IL-18 Rβ (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830727 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human IL-18 Rβ this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-18 Rβ is 1.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-545. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human IL-18 Rβ bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-18 Rβ (at 40 ng/ml in the presence of KG-1 cells at 1 x 106 cells/ml) is 0.3 - 1.2 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human IL-18 Rβ recognizes Human IL-18 Rβ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Beta chain of interleukin-18 receptor (IL-18Rbeta) is an accessory protein-like molecule that is required for signaling1 IL-18 signals through its specific cell surface receptor IL-18R, which comprises two subunits: IL-18Rα and IL-18Rβ. IL-18Rα alone has a weak affinity for IL-18 binding, while the IL-18Rα/β complex has a high affinity.2 IL-18 Rβ is detected in numerous tissues including lung, spleen, leukocytes and colon.3 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Churakowa, T. et al. (2000) J. Immunol. 165: 4950 2. Ghayur, T. et al. (2003) J. Immunol. 170: 5571 3. Born, TL. et al. (2000) J. Biol. Chem. 273: 29445 Technical ProtocolsN  Certificate of Analysis |
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I-604 | |
I-621 | |
I-545 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
