Anti-Human IL-21R (Clone 152504)
Anti-Human IL-21R (Clone 152504)
Product No.: I-720
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Clone 152504 Target IL-21R Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-21 Receptor, NILR Isotype IgG1 Applications ELISA Indirect , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Sf 21-Derived Recombinant Human Interleukin-21 Receptor Extracellular Domain (Accession # Q9HBE5) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830841 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human IL-21 R is approximately 25 ng/well. Western Blotting: To detect Human IL-21 R this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-21 R is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human Interleukin 21 Receptor (IL-21 R) (Clone 152504) recognizes an epitope on Human IL-21 R. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21 . The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family.1,2 IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling.3,4 Both IL-21R and the gc are necessary for the IL-21 function. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. PubMed NCBI Gene Bank ID UniProt.org References & CitationsTechnical Protocols  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
