Anti-Human IL-28A

Anti-Human IL-28A

Product No.: I-382


Target IL-28A Product Type Polyclonal Antibody Alternate Names Interleukin-28A, IFNL2 (IFN-Lambda-2 Protein), IFNλ2 Applications ELISA Indirect , ICC , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified Recombinant Human Interferon Lambda-1 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830658 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:G505. The detection sensitivity of this indirect ELISA for Human IL-28A is approximately 0.5 ng/well. Western Blotting: To detect Human IL-28A this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-28A is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-377. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human IL-28A bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-28A (at 0.1 ng/ml) is 0.3 - 1.5 µg/ml. Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Human Interleukin-28A (IL-28A) recognizes Human IL-28A. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Interleukin-28A (IL-28A) also known as IFNL2 is a novel cytokine that exerts its biological effects through a receptor that also binds IL28B and IL29. IL28A induces phosphorylation of STAT1, STAT2, and STAT3¹ IL-28A has antiviral activities and stimulates antiviral responses through a heterodimeric receptor. IL-28A represents a potential novel approach for therapy of Th1-mediated inflammatory diseases such as T-cell-mediated hepatitis.² PubMed IL-28A NCBI Gene Bank ID 282616 UniProt.org More Information on Uniprot.org References & Citations 1. Zitzmann, K. et al.(2006) Biochem. Biophys. Research Commun. 344:1334 2. Neurath, MF. et al.(2007) Gastroenterology 132:358 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols ICC N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human Interferon Lambda-1 (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830658

Applications and Recommended Usage?
Quality Tested by Leinco

Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:G505. The detection sensitivity of this indirect ELISA for Human IL-28A is approximately 0.5 ng/well.
Western Blotting: To detect Human IL-28A this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-28A is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-377.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human IL-28A bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-28A (at 0.1 ng/ml) is 0.3 - 1.5 µg/ml.
Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Interleukin-28A (IL-28A) recognizes Human IL-28A. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Interleukin-28A (IL-28A) also known as IFNL2 is a novel cytokine that exerts its biological effects through a receptor that also binds IL28B and IL29. IL28A induces phosphorylation of STAT1, STAT2, and STAT3¹ IL-28A has antiviral activities and stimulates antiviral responses through a heterodimeric receptor. IL-28A represents a potential novel approach for therapy of Th1-mediated inflammatory diseases such as T-cell-mediated hepatitis.²

PubMed

IL-28A

NCBI Gene Bank ID

282616

UniProt.org

More Information on Uniprot.org