Anti-Human IL-29 - Biotin - Leinco Technologies

Anti-Human IL-29 – Biotin

Product No.: I-383


Target IL-29 Product Type Polyclonal Antibody Alternate Names IFN-λ1, IFNL1 (IFN-Lambda1), λ1, Interleukin-29 Applications ELISA Det , WB


Select Product Size 50 µg — $590.00	Qty Max: Min: 1 Step: 1 $590.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified Recombinant Human IL-29 (Accession # Q8IU54) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830659 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human IL-29 this biotin conjugate can be used at a concentration of 0.1 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-29 is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-376. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:I-878 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human IL-29. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Human Interleukin-29 (IL-29) recognizes Human IL-29. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Interleukin-29 (IL-29), also known as IFN-lambda1, is a protein of the helical cytokine family and is a type III interferon.¹ It is also known as IFNλ1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 stimulates antiviral responses through a heterodimeric receptor.² It plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. PubMed IL-29 NCBI Gene Bank ID 282618 UniProt.org More Information on Uniprot.org References & Citations 1. Vilcek, J. et al.(2003) Nature Immunol. 4:8 2. Klucher, KM. et al.(2006) Hepatology 44:896 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols ELISA Det Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human IL-29 (Accession # Q8IU54)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830659

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human IL-29 this biotin conjugate can be used at a concentration of 0.1 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-29 is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-376.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:I-878 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human IL-29.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Interleukin-29 (IL-29) recognizes Human IL-29. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Interleukin-29 (IL-29), also known as IFN-lambda1, is a protein of the helical cytokine family and is a type III interferon.¹ It is also known as IFNλ1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 stimulates antiviral responses through a heterodimeric receptor.² It plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.

PubMed

IL-29

NCBI Gene Bank ID

282618

UniProt.org

More Information on Uniprot.org