Anti-Human IL-31 - Biotin - Leinco Technologies

Anti-Human IL-31 – Biotin

Product No.: I-420


Target IL-31 Product Type Polyclonal Antibody Alternate Names Interleukin-31 Applications ELISA Det , WB


Select Product Size 50 µg — $590.00	Qty Max: Min: 1 Step: 1 $590.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified Recombinant Human IL-31 (Accession # NP_001014358) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830679 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human IL-31 this biotin conjugate can be used at a concentration of 0.1 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-417. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human IL-31. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Human Interleukin-31 (IL-31) recognizes Human IL-31. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Interleukin-31 (IL-31) is a T-cell cytokine, which is preferentially produced by type 2 helper T cells. The structure of IL-31 places it in the IL-6 family of cytokines. IL-31 signals via a receptor complex that is composed of IL-31 receptor A and oncostatin M receptor subunits. IL-31 is believed to play a role in inflammation of the skin.¹ IL-31 represents a novel target for antipruritic drug development.² PubMed IL-31 NCBI Gene Bank ID 386653 UniProt.org More Information on Uniprot.org References & Citations 1. Dillon, SR. et al.(2004) Nature Immunol. 5:752 2. Homey, B. et al.(2006) J Allergy Clin Immunol. 117:411 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols ELISA Det Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human IL-31 (Accession # NP_001014358)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830679

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human IL-31 this biotin conjugate can be used at a concentration of 0.1 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-417.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human IL-31.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Interleukin-31 (IL-31) recognizes Human IL-31. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Interleukin-31 (IL-31) is a T-cell cytokine, which is preferentially produced by type 2 helper T cells. The structure of IL-31 places it in the IL-6 family of cytokines. IL-31 signals via a receptor complex that is composed of IL-31 receptor A and oncostatin M receptor subunits. IL-31 is believed to play a role in inflammation of the skin.¹ IL-31 represents a novel target for antipruritic drug development.²

PubMed

IL-31

NCBI Gene Bank ID

386653

UniProt.org

More Information on Uniprot.org

References & Citations

1. Dillon, SR. et al.(2004) Nature Immunol. 5:752
2. Homey, B. et al.(2006) J Allergy Clin Immunol. 117:411

View product citations for antibody PRODUCT_CODE on CiteAb

ELISA Det

Certificate of Analysis

Request COA

Prod No.	Description
I-503	Anti-Mouse IL-31 (NT)
I-502	Anti-Human IL-31 (Intermediate Domain)
I-419	Anti-Human IL-31
I-417	Recombinant Human IL-31
I-420	Anti-Human IL-31 – Biotin

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human IL-31 – Biotin