Anti-Human IL-4 (Clone 3010)
Anti-Human IL-4 (Clone 3010)
Product No.: I-173
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Clone 3010 Target IL-4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-4, BCGF, BCDF, B Cell Stimulating Factor, BSF-1 Isotype IgG1 Applications ELISA Cap , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human IL-4 (Accession # P05112.1) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830567 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 - 8 µg/ml. A suitable detection antibody is PN:I-251 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-186. Western Blotting: To detect Human IL-4 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-4 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 3010 recognizes an epitope on human IL-4. Background IL-4 is a 15-19 kD pleiotropic immune cytokine secreted by activated TH2 cells. It has many biological roles, including the regulation in humoral and adaptive immunity, the inhibition of bone resorption, the stimulation of activated B-cell and T-cell proliferation, and initiation of allergic responses. Moreover, IL-4 induces a positive feedback loop by stimulating differentiation of naive helper T cells, or Th0 cells, to Th2 cells which in turn secrete more IL-4. Increased IL-4 production and overproduction of IL-4R are found in many cancers. IL-4 has a significant effect on tumor progression by increasing apoptosis resistance via tumor growth enhancement. IL-4 may also play a part in the immune dysregulation that occurs in most HIV-1 infected patients. PubMed Research Area Other Molecules References & Citations1. Pike, JW. et al. (2001) PNAS 98: 2443 2. Finkelman, FD. et al. (2003) J Immunol. 170: 3835 3. Kaplan, MH. et al. (2008) J Immunol. 180: 3551 Technical Protocols  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
