Anti-Human IL-4 – Purified in vivo GOLD™ Functional Grade
Anti-Human IL-4 – Purified in vivo GOLD™ Functional Grade
Product No.: I-1163
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Clone MP4-25D2 Target IL-4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names BCGF, BCDF, B Cell Stimulating Factor, BSF-1 Isotype Rat IgG1 κ Applications CyTOF® , IHC , in vivo , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen CHO-expressed, recombinant human IL-4 Product Concentration ≥ 2.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2830503 Applications and Recommended Usage? Quality Tested by Leinco ELISA:Each lot of this antibody is quality control tested by ELISA. Additional Reported Applications For Relevant Conjugates ? N For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone MP4-25D2 recognizes human IL-4. Background IL-4 is a 15-19 kD pleiotropic immune cytokine secreted by activated TH2 cells. It has many biological roles, including the regulation in humoral and adaptive immunity, the inhibition of bone resorption, the stimulation of activated B-cell and T-cell proliferation, and initiation of allergic responses. Moreover, IL-4 induces a positive feedback loop by stimulating differentiation of naive helper T cells, or Th0 cells, to Th2 cells which in turn secrete more IL-4. Increased IL-4 production and overproduction of IL-4R are found in many cancers. IL-4 has a significant effect on tumor progression by increasing apoptosis resistance via tumor growth enhancement. IL-4 may also play a part in the immune dysregulation that occurs in most HIV-1 infected patients. PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Immunology . Neuroinflammation . Neuroscience . Other Molecules References & Citations1. Pike, JW. et al. (2001) PNAS 98: 2443 2. Finkelman, FD. et al. (2003) J Immunol. 170: 3835 3. Kaplan, MH. et al. (2008) J Immunol. 180: 3551 Technical ProtocolsCyTOF®   N  Certificate of Analysis |
Formats Available
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Prod No. | Description |
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I-1167 | |
I-1163 | |
I-1166 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
