Anti-Human IL-4 R alpha
Anti-Human IL-4 R alpha
Product No.: I-638
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Clone 25463 Target IL-4 R alpha Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-4 Receptor alpha, CD124, IL4RA Isotype IgG2a Applications CyTOF® , FC , ICC , IHC FFPE , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human IL-4 R (Accession # P24394) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830760 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-4R. A suggested method would be to stain cells expressing IL-4R with 0.25 µg per 1-2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:M1189. Western Blotting: To detect Human IL-4 R this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-4 R is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-186. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8-25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human IL-4R bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-4R (at 0.2 ng/ml) is 3-6 µg/ml. Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human Interleukin 4 Receptor (IL-4R) (Clone 25463) recognizes an epitope on Human IL-4R. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rhIL-9 R, rmIL-4 R, rhIL-5 Rα, rhIL-5 Rβ, rhIL-13 Rα1 or rhIL-13 Rα2 Background Interleukin 4 receptor (IL-4R) is a type I cytokine receptor that is expressed by lymphocytes and is well known for its role in immunomodulatory signaling and has also been documented on oligodendrocytes, suggesting involvement in glial cell interactions. IL-4R exerts a negative regulatory role on oligodendrocytes that when deleted results in enhanced myelin repair.1 IL-4R in brain tumors may serve as an attractive target for anticancer therapy.2 PubMed References & Citations1. Raine, CS. et al. (2008) Am J Pathol. 173(1):119-29. 2. Puri, RK. et al. (2001) Cancer Res. 61: 8058 3. Jiang, H. et al. (2000) J. Allergy Clin. Immunol. 105:1063 4. Hall, IP. et al. (2000) Respir. Res. 1:6 5. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701 Technical ProtocolsCyTOF®  ICC IHC FFPE N  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
