Anti-Human L-Selectin

CHO Cell-Derived Recombinant Human L-Selectin

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Blocking: This antibody shows inhibition in the LS180 adhesion assay. The adhesion of human LS80 cells (1 x 104 cells/well) to 10 μg/mL immobilized rhL-Selectin (100 μL/well) was maximally inhibited (80 - 100%) following pre-treatment with antibody for 1 hour using 100 μL of a 25 μg/mL antibody solution per well.

Sheep Anti-Human L-Selectin recognizes Human L-Selectin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain¹ found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.²

1. Levy, K. et al.(2005) J. Cell Mol. Med. 9: 255
2. Spangrude, GJ. et al.(2004) Blood 103: 2990