Anti-Human Leptin (Clone 44802)

E. coli-Derived Recombinant Human Leptin (Accession # P41159)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Human OB bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human OB (at 7.5 ng/ml) is 0.4 - 1.6 µg/ml.

Mouse Anti-Human Leptin (Clone 44802) recognizes an epitope on Human Leptin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Leptin is a neuroendocrine hormone secreted from adipose tissue that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. Leptin is one of the most important adipose derived hormones.¹ It interacts with six types of receptors and once it binds to OB-Receptor it activates the stat3, which is phosphorylated and travels to the nucleus to effect changes in gene expression. One of the main effects on gene expression is the down-regulation of the expression of endocannabinoids.² High leptin levels are associated with psoriasis.³

1. Mantzoros, CS. et al.(2006) Nat Clin Pract Endocrinol Metab. 2: 318
2. Hill, RA. et al.(2002) Int. J. Obes. Relat. Metab. Disord. 26: 1407
3. Chen, YJ. et al.(2008) Arch Derm. 144: 1571