Anti-Human LIF

E. coli-derived recombinant human LIF (Acession No.: P15018)

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

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IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml.
Neutralization: Measured by its ability to neutralize LIF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.04-0.08 µg/mL in the presence of 3 ng/mL Recombinant Human LIF.

Goat Anti-Human Leukemia Inhibitory Factor recognizes Human LIF by ELISA. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. By ELISAs, less than 50% cross-reactivity with recombinant mouse LIF was observed.

Leukemia inhibitory factor (LIF) (22 kDa (predicted) glycoprotein) is a pleiotropic cytokine that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190, and the subunit gp130. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha -chain and a 130 kDa signal-transducing beta -chain (gp130), which is shared with the other members of the IL-6 family.

Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells.