Anti-Human Mcl-1 (Intermediate Domain)

PN:M1216

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human Mcl-1 recognizes an epitope in the intermediate domain of Human Mcl-1. This polyclonal antibody was purified using affinity chromatography.

Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival (reviewed in 1). While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak.^2,3 Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals.³ Mcl-1 exists as at least two distinct isoforms designated Mcl-1L and Mcl-1S. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.

1. Edwards, SW. et al. (2004) Biochem Soc Trans. 32:489
2. Cuconati, A. et al. (2003) Genes and Dev. 17:2922
3. Opferman, JT. et al. (2003) Nature 426:671
4. Kozopas, KM. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3516