Anti-Human MCP-2

Purified Recombinant Human MCP-2 (>98%)

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: Measured by its ability to neutralize CCL8/MCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 1.5-5.0 µg/mL in the presence of 250 ng/mL Recombinant Human CCL8/MCP‑2.

Goat Anti-Human Monocyte Chemotactic Protein-2 (MCP-2) recognizes Human MCP-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Monocyte chemotactic protein-2 (MCP-2) is a non-glycosylated polypeptide that is a member of the C-C chemokine subfamily and it specifically attracts monocytes, lymphocytes, basophils and eosinophils. It also plays a role in neoplasia and inflammatory host responses. MCP-2 can bind heparin.¹

1. Van Damme, J. et al. (1998) J. Immunol. 160: 4034