Anti-Human MCP-3

Purified Recombinant Human MCP-3 (>98%)

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: Measured by its ability to neutralize CCL7/MCP‑3/MARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 10-30 µg/mL in the presence of 1 µg/mL Recombinant Human CCL7/MCP‑3/MARC.

Goat Anti-Human Monocyte Chemotactic Protein-3 (MCP-3) recognizes Human MCP-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Monocyte chemotactic protein-3 (MCP3) is a non-glycosylated polypeptide belonging to the the CC-Chemokines family that attracts monocytes and eosinophils, augments monocyte anti-tumor activity, and induces the release of gelatinase B. It also regulates protease secretion by macrophages. MCP3 can bind heparin.¹ MCP-3 is one of the most pluripotent chemokines since it activates all types of leukocytes.
MCP-3 may affect HIV-1 infection via inhibiting the binding of HIV envelope to CCR5.² MCP-3 has also been implicated in Multiple Sclerosis, allergic reactions and viral infections.^3,4,5

1. Van Damme, J. et al. (1993) Biochem. Biophys. Res. Commun. 191: 535
2. Parmentier, M. et al. (1999) Blood 94: 1899
3. Brosnan, CF. et al. (1998) J Neuroimmunol. 86: 20
4. Reddigari, SR. et al. (1995) Exp. Dermatol. 4: 260
5. Matikainen, S. et al. (2001) Cytokine Growth Factor Rev. 12: 171