Anti-Human Midkine

Product No.: M133


Target Midkine Product Type Polyclonal Antibody Alternate Names MDK, FLJ27379, NEGF2 (Neurite Growth-Promoting Factor 2) Applications IHC FFPE , WB


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Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified Recombinant Human Midkine (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831316 Applications and Recommended Usage? Quality Tested by Leinco Western Blot: Suitable for use at concentration of 2 µg/mL. Additional Applications Reported In Literature ? Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Rabbit Anti-Human Midkine recognizes Human Midkine. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Midkine (MK, MDK), or Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor¹ , a member of the NEGF family founded by Pleiotrophin. It is a non-glycosylated protein. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. MK application could be a new therapeutic strategy for the treatment of ischemic heart failure.² PubMed Midkine NCBI Gene Bank ID 4192 UniProt.org More Information on Uniprot.org References & Citations 1. Kodama, I. et al. (2006) Circulation 114: 1713 2. Kenji, K. et al. (2003) Am J Physiol Heart Circ Physiol. 296: H462 View product citations for antibody M133 on CiteAb Technical Protocols IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human Midkine (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831316

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blot: Suitable for use at concentration of 2 µg/mL.

Additional Applications Reported In Literature ?

Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rabbit Anti-Human Midkine recognizes Human Midkine. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Midkine (MK, MDK), or Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor¹ , a member of the NEGF family founded by Pleiotrophin. It is a non-glycosylated protein. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. MK application could be a new therapeutic strategy for the treatment of ischemic heart failure.²

PubMed

Midkine

NCBI Gene Bank ID

4192

UniProt.org

More Information on Uniprot.org