Anti-Human Midkine - Biotin - Leinco Technologies

Anti-Human Midkine – Biotin

Product No.: M135


Target Midkine Product Type Polyclonal Antibody Alternate Names MDK, FLJ27379, NEGF2 (Neurite Growth-Promoting Factor 2) Applications WB


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Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified Recombinant Human MK (Accession # P21741) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831318 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human MK this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human MK is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M132. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Human Midkine (MK) recognizes Human MK. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Midkine (MK, MDK), or Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor¹ , a member of the NEGF family founded by Pleiotrophin. It is a non-glycosylated protein. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. MK application could be a new therapeutic strategy for the treatment of ischemic heart failure.² PubMed Midkine NCBI Gene Bank ID 4192 UniProt.org More Information on Uniprot.org References & Citations 1. Kodama, I. et al. (2006) Circulation 114: 1713 2. Kenji, K. et al. (2003) Am J Physiol Heart Circ Physiol. 296: H462 View product citations for antibody M135 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human MK (Accession # P21741)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831318

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human MK this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human MK is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M132.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Midkine (MK) recognizes Human MK. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Midkine (MK, MDK), or Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor¹ , a member of the NEGF family founded by Pleiotrophin. It is a non-glycosylated protein. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. MK application could be a new therapeutic strategy for the treatment of ischemic heart failure.²

PubMed

Midkine

NCBI Gene Bank ID

4192

UniProt.org

More Information on Uniprot.org