Anti-Human MIP-1α – Clone 93342
Anti-Human MIP-1α – Clone 93342
Product No.: M154
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Clone 93342 Target MIP-1 alpha Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CCL3, LD78Alpha, MIP1A, SCYA3 Isotype IgG2b Applications CyTOF® , FC , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human MIP-1α (Accession # P10147) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831358 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MIP-1α. A suggested method would be to stain cells expressing MIP-1α with 2.5 µg/106 cells in a total staining volume of ≤200 µl followed by PN:M1189. Western Blotting: To detect Human MIP-1α this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MIP-1α is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L145. Additional Applications Reported In Literature ? CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human Macrophage Inflammatory Protein 1 Alpha (MIP-1α) (Clone 93342) recognizes an epitope on Human MIP-1α. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background MIP-1α is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.1 MIP-1α is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.2 The role of MIP-1α seems to be related to production of TNF-α, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.3 PubMed References & Citations1. Pragnell, IB. et al. (1994) J. Biol. Chem. 269: 4974 2. Cook, DN. et al. (1996) J. of Leukocyte Biol. 59: 61 3. Ward, PA. et al. (1995) J of Immunol. 154: 4793 Technical ProtocolsCyTOF®   Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
