Anti-Human MIP-1α – Clone 93321

Purified Recombinant Human MIP-1α (Accession # P10147)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Human CCL3 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CCL3 (at 100 ng/ml) is 0.5-1.5 µg/ml.
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.

Mouse Anti-Human Macrophage Inflammatory Protein 1 Alpha (MIP-1α) (Clone 93321) recognizes an epitope on Human MIP-1α. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Macrophage inflammatory protein 1 alpha (MIP-1 alpha) is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.¹ MIP-1 alpha is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.² The role of MIP-1 alpha seems to be related to production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.³

1. Pragnell, IB. et al. (1994) J. Biol. Chem. 269: 4974
2. Cook, DN. et al. (1996) J. of Leukocyte Biol. 59: 61
3. Ward, PA. et al. (1995) J of Immunol. 154: 4793