Anti-Human MIP-1β – Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human MIP-1β (>98%) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831370 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human MIP-1β this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human MIP-1β is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M162. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:M1270 as the capture antibody at 2 - 8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human MIP-1β. Additional Applications Reported In Literature ? Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rabbit Anti-Human Macrophage Inflammatory Protein-1β (MIP-1β) recognizes Human Macrophage Inflammatory Protein-1β. This antigen affinity purified polyclonal antibody was purified using immobilized antigen agarose chromotography. Background Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. PubMed NCBI Gene Bank ID References & CitationsTechnical ProtocolsELISA Det ICC  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
