Anti-Human/Mouse Bax

Sf 21-Derived Human Bax Minus The Carboxyl Terminal

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

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Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.

Mouse Anti-Human Bax (Clone 127602) recognizes an epitope on Human Bax. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer.1 The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death.2,3 Bax, a pro-apoptotic Bcl-2 family member, is a cytosolic protein that changes conformation and translocates to the mitochondria following apoptotic stimuli.4,5 It is thought to share significant functional homology with Bak, another pro-apoptotic Bcl-2 family member, as disruption of bax or bak has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking both show decreased apoptotic capability.