Anti-Human/Mouse BMPR-IB/ALK-6
Anti-Human/Mouse BMPR-IB/ALK-6
Product No.: B580
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Clone 88614 Target BMPR-IB Formats AvailableView All Product Type Monoclonal Antibody Alternate Names ALK-6, CDw293, Bone Morphogenetic Protein Receptor IB Isotype IgG2a Applications WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human BMPR-IB Extracellular Domain Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828250 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human BMPR-IB this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human BMPR-IB is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human Bone Morphogenetic Protein Receptor IB (BMPR-IB) (Clone 88614) recognizes an epitope on Human BMPR-IB. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows 100% cross-reactivity with rmBMPR-IB and no cross-reactivity with rhBMPR-IA, rmBMPR-IA or rhBMPR-II. Background Bone morphogenetic proteins (BMP) are members of the transforming growth factor beta (TGF-beta) superfamily and are involved in a wide variety of biological processes, including osteoblast differentiation and bone healing. The activities of the BMP are mediated by signal transduction via three BMP receptors (BMPR-IA, -IB and -II), which are thus essential for the biological actions of the BMP. Although the precise mechanisms which control the BMPR are not yet known, it is possible that post-translational regulation of these cell surface antigens by shedding could modulate their expression and thereby at least partly determine the response of the cells to the BMP. The BMPR1B receptor plays a role in the formation of middle and proximal phalanges. BMPR1B has also been designated as CDw293. Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. Human and mouse BMPR-IB are highly conserved and share 98% sequence identity. PubMed References & CitationsTechnical Protocols Certificate of Analysis |
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