Anti-Human/Mouse integrin β7 (Clone FIB21) – Purified

TK1 cells

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This purified antibody is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

Anti-Human/Mouse integrin β7 activity is directed against human and mouse β7 integrin.

Integrins are a large family of heterodimeric transmembrane molecules that mediate adhesion, migration, cell survival, and cell differentiation. β7 can pair with CD49d to form the heterodimeric integrin receptor α4β7 or with CD103 to form αEβ7. α4β7 integrin is a lymphocyte receptor for the mucosal vascular addressin MADCAM-1¹. αEβ7 is an α-I domain-containing integrin that is highly expressed by a variety of leukocyte populations at mucosal sites including dendritic cells, intraepithelial T cells, mast cells, and T regulatory cells.

FIB21 was generated by immunizing Wister rats with TK1 murine T cell lymphoma cells². Spleen cells were then fused with Sp2/0-Ag14 myeloma cells to create a hybridoma line. FIB21 is known to block adhesion to MADCAM-1 and E-cadherin as well as α4β7 integrin binding to VCAM-1 and fibronectin. FIB21 maps to amino acid resides 176-250, which lie within the region of β7 that specifies MADCAM-1 binding³.

Integrin β7 heterodimer α4/β7 is expressed on peripheral lymphocytes, thymocytes and bone marrow progenitors. Integrin β7 heterodimer αE/β7 is expressed on intestinal IEL, dendritic epidermal T cells, T regulatory cells, CD8+ T cells in lymph nodes and lamina propria.

CD49d/ß7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/ß7: E-cadherin

Lymphocyte adhesion, hematopoietic progenitor cells migration

1. Berlin C, Berg EL, Briskin MJ, et al. Cell. 74(1):185-195. 1993.
2. Andrew DP, Berlin C, Honda S, et al. J Immunol. 153(9):3847-3861. 1994.
3. Tidswell M, Pachynski R, Wu SW, et al. J Immunol. 159(3):1497-1505. 1997.
4. Meerschaert J, Vrtis RF, Shikama Y, et al. J Immunol. 163(11):6217-6227. 1999.
5. Higgins JM, Cernadas M, Tan K, et al. J Biol Chem. 275(33):25652-25664. 2000.