Anti-Human/Mouse Mac-2 (Galectin-3) (Clone M3/38 ) – Purified No Carrier Protein

Raised against galectin-3 of mouse origin

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

ELISA,
FC,
IF,
IHC,
IP,
WB

M3/38 activity is directed against human and mouse Mac-2 (Galectin-3).

Mac-2 (Galectin-3) is a β-galactoside-binding lectin of the chimera-type, characterized by a single carbohydrate recognition domain and an N-terminal polypeptide tail region¹. Mac-2 plays important roles in cell proliferation, apoptotic regulation, cell-to-cell and cell-to-matrix interactions, macrophage activation, angiogenesis, inflammation, fibrosis and host defense. Additionally, Mac-2 is an early-stage diagnostic and/or prognostic biomarker for viral infection, neurodegenerative disorders, tumor formation, diabetes mellitus, and certain types of heart, kidney, and autoimmune disease. The primary structure of mouse and human Mac-2 is highly conserved with 85% similarity². Human Mac-2 binds to asialofetuin and purified laminin.

M3/38 was generated by immunizing rats with immunoadsorbent-purified macrophage glycoproteins and fusing the resulting spleen cells with mouse myeloma cells³. M3/38 is able to immunoprecipitate both the mouse and human Mac-2 homologs^2,3. M3/38 binding to Mac-2 can be inhibited by galactose². M3/38 recognizes a linear epitope within the N-terminal domain of Mac-2⁴. The common minimal sequence as determined by SPOT analysis as Ala-Pro-Pro-Gly-Ala-Tyr.

M3/38 has been exploited for live cell thyroid tumor imaging^4,5. A chimeric M3/38 Fab-fragment and its humanized derivative can detect Mac-2 overexpression in cancerous thyroid nodules in mice via positron emission tomography. The three-dimensional structure of the rat/human chimeric Fab has been determined⁵.

Mac-2 (Galectin-3) is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Mac-2 is secreted into fluids such as serum and urine as well as released by injured and inflammatory cells. Mac-2 was originally identified in murine inflammatory macrophages. Mac-2 is also expressed by various cell lines (e.g., HeLa, SL68, HL60, HT29).

P17931

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