Anti-Human/Mouse/Rat β-Catenin 1 (Clone 12F7) – Purified (PhenoCycler-Fusion (CODEX)® Ready)

Clone 12F7 has been validated for use on PhenoCycler® using [Mouse/Human] [Tissue] [Tissue Type either FFPE or FF] tissue.

N-terminal fragment of β-catenin fused to MBP

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.

This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

IHC
IF
WB
FC

12F7 activity is directed against human, mouse, and rat β-catenin 1.

β-catenin 1 is widely expressed in many tissues, including kidney, lung, adult and developing central nervous system, several hair follicle cell types, colon, cortical neurons, and various cancers.

β-catenin is an armadillo protein that binds to numerous partners, including cadherins, adenomatosis polyposis coli, and T-cell factor ¹ , and is also a principal component of the Wnt signaling pathway ² . β-catenin is necessary for embryonic development, organ differentiation and development, bone homeostasis, mammalian hair growth, and adult tissue regeneration. Additionally, β-catenin functions in cell adhesion as part of a cadherin/catenin complex, plays an important role in the structural integrity of squamous epithelial cells ³ , and is involved in synaptic plasticity ¹ . β-catenin localizes to the cell cytoplasm, and once activated, translocates to the nucleus, where it activates target genes via TCF/LEF transcription factors . Abnormal signaling leads to disease, including cancer, lung disease (e.g., chronic obstructive pulmonary disease), atherosclerosis, and Alzheimer’s Disease.

12F7 was generated by immunizing Balb/c mice with a purified in-frame fusion protein of β- catenin bound to maltose binding protein ⁴ . The β-catenin was derived from a 2.8 kb cDNA clone isolated from a chicken embryo cDNA library, of which a 2.4 kb section encoding residue 92 through the carboxy terminus was used. Half the splenic lymphocytes were fused with the P3-X63Ag8.653 myeloma cell line and the other half with Sp2/0-Ag14. The 12F7 binding epitope was mapped to a region between residues 112 and 134 via a deletion strategy. 12F7 does not cross-react with plakoglobin.

β-catenin localizes to cell-cell contacts (adherens junctions) and the cell membrane, as well as the cytoplasmic pool of protein.

1. Maguschak KA, Ressler KJ. Neuropharmacology. 62(1):78-88. 2012.
2. Liu J, Xiao Q, Xiao J, et al. Signal Transduct Target Ther. 7(1):3. 2022.
3. Politi EN, Lazaris AC, Kehriotis M, et al. Cytopathology. 19(3):172-178. 2008.
4. Johnson KR, Lewis JE, Li D, et al. Exp Cell Res. 207(2):252-260. 1993.
5. Sacco PA, McGranahan TM, Wheelock MJ, et al. J Biol Chem. 270(34):20201-20206. 1995
6. Radice GL, Rayburn H, Matsunami H, et al. Dev Biol. 181(1):64-78. 1997.