Anti-Human/Mouse/Rat β-Catenin 1 (Clone 12F7) – Purified No Carrier Protein

N-terminal fragment of β-catenin fused to MBP

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

IHC
IF
WB
FC

12F7 activity is directed against human, mouse, and rat β-catenin 1.

β-catenin is an armadillo protein that binds to numerous partners, including cadherins, adenomatosis polyposis coli, and T-cell factor ¹ , and is also a principal component of the Wnt signaling pathway ² . β-catenin is necessary for embryonic development, organ differentiation and development, bone homeostasis, mammalian hair growth, and adult tissue regeneration. Additionally, β-catenin functions in cell adhesion as part of a cadherin/catenin complex, plays an important role in the structural integrity of squamous epithelial cells ³ , and is involved in synaptic plasticity ¹ . β-catenin localizes to the cell cytoplasm, and once activated, translocates to the nucleus, where it activates target genes via TCF/LEF transcription factors . Abnormal signaling leads to disease, including cancer, lung disease (e.g., chronic obstructive pulmonary disease), atherosclerosis, and Alzheimer’s Disease.

12F7 was generated by immunizing Balb/c mice with a purified in-frame fusion protein of β- catenin bound to maltose binding protein ⁴ . The β-catenin was derived from a 2.8 kb cDNA clone isolated from a chicken embryo cDNA library, of which a 2.4 kb section encoding residue 92 through the carboxy terminus was used. Half the splenic lymphocytes were fused with the P3-X63Ag8.653 myeloma cell line and the other half with Sp2/0-Ag14. The 12F7 binding epitope was mapped to a region between residues 112 and 134 via a deletion strategy. 12F7 does not cross-react with plakoglobin.

β-catenin 1 is widely expressed in many tissues, including kidney, lung, adult and developing central nervous system, several hair follicle cell types, colon, cortical neurons, and various cancers.

β-catenin localizes to cell-cell contacts (adherens junctions) and the cell membrane, as well as the cytoplasmic pool of protein.

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