Anti-Human MSP – Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen NSO-Derived MSP (Accession # AAA59872) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831301 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human MSP this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human MSP is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Macrophage Stimulating Protein (MSP) recognizes Human MSP. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Macrophage-stimulating protein (MSP), also known as hepatocyte growth factor-like, is serum protein with homology to hepatocyte growth factor (HGF).1 MSP is a soluble cytokine that belongs to the family of the plasminogen-related growth factors (PRGFs). PRGFs are alpha/beta heterodimers that bind to transmembrane tyrosine kinase receptors. Through binding to its receptor, MSP stimulates dissociation of epithelia and works as an inflammatory mediator by repressing the production of nitric oxide (NO).2 MSP exerts proliferative and antiapoptotic effects, suggesting that it may play a role in tubular regeneration after acute kidney injury.3 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Suda, T. et al. (1997) J Clin. Invest. 9: 701 2. Paolo, M. et al. (2001) Molecular Biol. Of Cell 12: 1341 3. Camussi, G. et al. (2008) J Am Soc Nephrol. 19: 1904 Technical Protocols Certificate of Analysis |
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