Anti-Human Nanog

Purified Recombinant Human Nanog (>98%)

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Rabbit Anti-Human Nanog recognizes Human Nanog. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Nanog, a homeobox transcription factor¹, plays a key role in maintaining stem cell pluripotency by regulating the expression of genes critical to stem cell renewal and differentiation.² In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm). Nanog may be useful in the immunohistochemical diagnosis of tumors. It is expressed in germ cells of the fetus and in some germ cell tumors of the gonads³ and central nervous system.⁴

1. Schöler, HR.et al. (2003) Cell 113:551
2. Pei, D. et al. (2005) J Biol. Chem. 280:1401
3. Rajpert-De Meyts, E. et al. (2005) Histopathology 47:48
4. Ligon, KL. et al. (2006) Am J Surg Pathol. 30:1613