Anti-Human Nanog – Biotin

Purified Recombinant Human Nanog (Accession # ABZ92376)

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.

Goat Anti-Human Nanog recognizes Human Nanog. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Nanog, a homeobox transcription factor¹, plays a key role in maintaining stem cell pluripotency by regulating the expression of genes critical to stem cell renewal and differentiation.² In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm). Nanog may be useful in the immunohistochemical diagnosis of tumors. It is expressed in germ cells of the fetus and in some germ cell tumors of the gonads³ and central nervous system.⁴

1. Schöler, HR.et al. (2003) Cell 113:551
2. Pei, D. et al. (2005) J Biol. Chem. 280:1401
3. Rajpert-De Meyts, E. et al. (2005) Histopathology 47:48
4. Ligon, KL. et al. (2006) Am J Surg Pathol. 30:1613