Anti-Human PUMA Antibody (CT) – p53 Upregulated Modulator of Apoptosis

PN:P156

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human p53 Upregulated Modulator of Apoptosis (PUMA) recognizes an epitope near the C-terminus of Human and Mouse PUMA. This polyclonal antibody was purified using affinity chromatography.

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse.^1-3 PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β.¹ PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

1. Nakano, K. et al. (2001) Mol Cell. 7(3):683
2. Han, J. et al. (2001) Proc. Natl. Acad. Sci. USA 98(20):11318