Anti-Human PDGF Rβ (Clone 61520)

Purified Recombinant Human PDGF Rβ (Accession # P09619)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

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Mouse Anti-Human PDGF Rβ recognizes an epitope on Human PDGF Rβ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. No cross-reactivity was observed with rmPDGF Rβ, rhPDGF Rα, rhgp130 or rhIL-6 R.

Platelet-derived growth factor receptor-β (PDGFRβ) is a well-characterized plasma membrane receptor with endogeneous tyrosine kinase activity, which is autophosphorylated in response to binding of dimeric platelet-derived growth factor (PDGF) ligand.¹ PDGFRβ mediates signaling important for the proliferation, migration, and survival of mesenchymal cells.² PDGFRβ is found in all cell types ³ and is activated in breast carcinoma cells and indicate a possible role of the receptor in breast cancerogenesis.⁴

1. Gilliland, DG. et al. (1996) Proc Natl Acad Sci USA 93: 14845
2. Ronnstrand, L. et al. (1998) Biochim Biophys Acta 1378: F79
3. Fischer, B. et al. (1997) Acta Anatomica 159: 194
4. Valius, M. et al. (2005) Biologija 1: 61